MOG-induced EAE is induced in DA or Lewis rats with protein emulsified in Incomplete Freunds Adjuvant (IFA) or Complete Freunds Adjuvant (CFA) respectively, and are models well suited for studies of immune mechanisms relevant to MS. MOG-induced EAE is a severe and demyelinating encephalomyelitis with a chronic progressive disease course. The disease is CD4+ T cell mediated and dependent on both Th1 and Th17 cells. T cells, B cells and macrophages are recruited to the inflammatory site resulting in demyelination and axonal loss. IL-17 and IFN-g responses from LNs cells can be assayed ex vivo as estimation on drug efficacy in vivo or prediction of efficacy for selection of lead compounds before in vivo experiments. The CD4+ T cell dependency of this model allows for adoptive T cell transfer following ex vivo stimulation of autoreactive T cells.