Customised Services in Preclinical Inflammation

PLP Induced EAE

Myelin proteolipid protein (PLP) induced EAE in SJL mice results in a relapsing-remitting disease, suitable for studies of efficacy. The encephalogenic peptide aa 139-151 is emulsified in adjuvant and injected s.c. Disease can be boosted with injection of pertussis toxin. Mice develop a relapsing EAE with ascending flaccid paralysis around 1-2 weeks after immunization. Autoreactive T cells are activated in the periphery and migrate into the CNS across the blood-brain-barrier. Upon entry into the CNS, T cells are re-activated by antigen-presenting cells, ultimately resulting in demyelination and axonal cell death. PLP-induced EAE is a severe and demyelinating encephalomyelitis with a relapsing-remitting disease course. The disease is CD4+ T cell mediated and dependent on both Th1 and Th17 cells. T cells, B cells and macrophages are recruited to the inflammatory site resulting in demyelination and axonal loss. IL-17 and IFN-g responses from LNs cells can be assayed ex vivo as estimation of drug efficacy in vivo or prediction of efficacy for selection of lead compounds before in vivo experiment.

PLP Induced